That's why we're pleased to launch the color-enhanced Blu10 Plus (BLUltra) prestained protein standards.Īs you may see the comparison data shown below, Blu10 Plus (BLUltra) shows a better resolution than the Blu12 (BLUeye) & Blu10 (BlueRAY) for the protein bands from 95 kDa to 270 kDa. Nowadays, more and more researchers are studying the high molecular weight proteins, meaning they may need high-resolution prestained protein standards, especially with high molecular weights. Additional data should be considered for a more accurate adjustment. Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Protein Stains Stains to visualize and quantify protein bands on gels and blots: Coomassie stains, highly sensitive fluorescent stains, silver stains, negative stains, and total protein blot stains. 2.5 μl per well for general Western transferring. Western blotting products include protein transfer systems, blotting membranes, filter paper, premixed blotting buffers and reagents, and detection kits. Migration patterns of Blu10 Plus Prestained Protein Ladder (BLUltra) in different electrophoresis conditions are listed below :ģ μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Guide for Molecular Weight Estimation (kDa) The Blu10 Plus Prestained Protein Ladder (BLUltra) keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes. The 10 recombinant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. The Blu10 Plus Prestained Protein Ladder (BLUltra) is a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa.
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